Digital breast tomosynthesis (DBT) is a promising new technique for breast cancer diagnosis. DBT has the potential to overcome the tissue superimposition problems that occur on traditional mammograms for tumor detection. However, DBT generates numerous images, thereby creating a heavy workload for radiologists. Therefore, constructing an automatic computer-aided diagnosis (CAD) system for DBT image analysis is necessary. This study compared feature-based CAD and convolutional neural network (CNN)-based CAD for breast cancer classification from DBT images. The research methods included image preprocessing, candidate tumor identification, three-dimensional feature generation, classification, image cropping, augmentation, CNN model design, and deep learning. The precision rates (standard deviation) of the LeNet-based CNN CAD and the feature-based CAD for breast cancer classification were 89.84 (0.013) and 84.46 (0.082), respectively. The T value was -4.091 and the P value was 0.00 < 0.05, which indicate that the LeNet-based CNN CAD significantly outperform the feature-based CAD. However, there is no significantly differences between the LeNet-based CNN CAD and the feature-based CAD on other criteria. The results can be applied to clinical medicine and assist radiologists in breast cancer identification.

Orange juice has become one of the most widely accepted natural beverages due to its invigorating flavours, source of quick energy in the form of natural sugars and its nutrient which support the growth of acid tolerant bacteria, yeasts and moulds. Present investigation was conducted to study the microbiological examination of commercially and freshly prepared orange juices. Freshly prepared orange juices were pasteurised, carbonated, concentrated and chemically treated with a preservative (sodium metabisulphite) and stored under, freezer, refrigeration and ambient temperature for 90 days. The pH ranged from 3.0 to 4.5 for the commercially, fresh and stored samples respectively. The total bacterial load ranged from 1.6x105 to 3.6x106 and the total yeast cell counts ranged from 1.7x104 to 4.8x106 cfu/ml for the commercially, freshly and stored samples. Twenty-two microbial species including 5 bacterial isolates, 6 yeast isolates and 11 isolates of mould were isolated from the orange juices. The bacterial isolates identified from the samples were Bacillus megaterium, Bacillus cereus, Bacillus pantothenticus, Bacillus aeruginosa and Escherichia coli. The fungal isolates were Saccharomyces cerevisiae, Saccharomyces rouxii, Saccharomyces telluris, Blastomyces sp, Aspergillus sp, Mycelia sp, Chrysosporium sp, and Trichoderma sp. Among the bacterial isolates, Bacillus sp was the predominant, while the fungi Saccharomyces sp and Mycelia sp were the predominant fungi and accounted for five out of 11 isolates. The isolates of bacteria, yeasts and moulds appear to be persistent throughout the period of this study and could be used as indicators of microbial quality. A safe microbial load and reduction in contamination of orange juice /fruit juices can be achieved by combination of processing methods such as chemical preservatives with pasteurisation,concentration with carbonation under controlled microbiological environments such as freezer and refrigeration temperatures.

Background: Presently, there are wide uses of detergents, which discharged to marine environment (Mediterrean sea). Detergents are organic chemical compounds, consisting of fatty acids, and different components of Alkyl Benzene, Sulphonates, Polyphosphates, Cellulose, Sucrinic acid, and Sodium Lauryl Sulfates. Further, even if at low concentrations, the synthetic detergents are considered to be toxic to marine biodiversity. Since, these surfactants are not completely degraded by waste water treatment. It has ability to damage the DNA and accumulated on fish and other marine organisms. Methods: Flow cytometry technique was applied, because it’s accuracy for analysis of DNA contents. Hence, the present study evaluated the impact of sodium lauryl sulfate as synthetic detergent on the DNA of sardine fish as a marine biological indicator. Sardinella aurita was collected from clean and SLS-contaminated sites. The flow cytometry technique was employed to assess levels of active (apoptosis) and passive (necrosis) cell death in both exposed and unexposed fishes to SLS. Result: Chemical detergent contains 120 ml (i.e., 20mg/L) of sodium lauryl sulfates. The result shows more distinct and significant differences of the early and late apoptosis/ necrosis of Sardine fish liver, compared with unexposed fish samples from cleaned reference site. Conclusions: The present data indicate that SLS was able to cause toxicity to fish. Data recorded revealed that toxicity as a significant accumulation of hepatic tissue cellular DNA during the G0-G1 in a significant way (P<0.05), compared with that of unexposed liver tissue. In the same way, there was an insignificant accumulation of hepatic cell’s DNA in the S phase but not during the G2/M phase, finally there was a significant arrest / DNA accumulation during the PreG1- phase (P<0.05). In the meantime, accompanies of apoptotic profile of cells exposed to SLS showed a significant total apoptotic form significantly elevated compared with reference site.

Mineral oil in metal containments with access of outside air may become contaminated with humidity present in the air because water starts to condense on cold metallic surfaces. An approach for the removal of this water moiety would be to remove it with polysaccharides that have the property of swelling up by water uptake. The polysaccharides can be fixed to carriers such as expanded clay particles. When oil containing water comes in contact with the polysaccharide coat, the water is taken up; the coated carriers containing water can be removed. A device is described that may allow application of this principle.

The purpose of this research was to determine the quality of mineral and fatty acid profile of imported horse mackerel Trachurus murphyi using the improved NIOMR fish smoking kiln. 20kg of frozen imported horse mackerel were obtained from Ijora frozen food market Lagos state, Nigeria. Samples were transported to the NIOMR Pilot plant for processing and commencement of chemical analysis. Results show that, the most abundant saturated fatty acid in the fish was Palmitic acid, with a value of 19.404%, while the most abundant monounsaturated fatty acid was Elaidic acid with a value of 12.63%. However, low values of polyunsaturated fatty acid was recorded in the fish species, and the most abundant polyunsaturated fatty acid is the Docosahexaenoic acid with a value of 2.297%. The most abundant polyunsaturated fatty acid recorded is Linoleic acid (2.075 while the least abundant polyunsaturated fatty acid was eicosapentaenoic acid (EPA, C20:5n-3). There was significant variation (p < 0.05) in the level of Docosahexaenoic acid and Eicosapentaenoic acid in the fish species. The monounsaturated fatty acids (MUFA) content of horse mackerel was mainly omega-9 fatty acids except Palmitoleic acid which is an omega-7 fatty acid. It can be concluded that the horse mackerel has a good mineral and fatty acid profiling.

The production of aroma in aromatic rice is due to the increase of 2-acetyl-1-pyrroline (2-AP) precursor substances caused by the functional deletion of Badh2 gene on chromosome 8, and the accumulation of 2AP makes rice produce aroma. In this study, Badh2 gene was isolated and cloned from 18 representative aromatic rice cultivars in Southern Henan, and the bioinformatics analysis of Badh2 gene was carried out. Meanwhile, seven functional molecular markers developed by Badh2 gene were used to detect and analyze Badh2 gene in 18 aromatic rice varieties from Southern Henan. The results showed that the coding region of Badh2 gene was 1509 bp in length. It contained 15 exons and 14 introns, and encoded 503 amino acids. There are many types of variation of the Badh2 gene in the 18 aromatic rice varieties. According to the variation of Badh2 gene, the tested aromatic rice varieties could be divided into three groups, among which Xinxianggeng 1, Xiangnuo 25, Heixiangdao 193 and Xiangbao 2 were concentrated in group ?, while the other 14 kinds of aromatic rice were concentrated in group II. Seven functional molecular markers of Badh2 gene were used to detect different varieties mutation types in exon 2, exon 4~5, exon 7 and exon 13 of Badh2 gene. No aromatic rice varieties with different mutation types were found in promoter region, exon 12 and exon 14 of Badh2 gene. Therefore, our results provide important information for understanding the genetic basis of fragrant genes in aromatic rice germplasm resources in Southern Henan and breeding new varieties of high-quality aromatic rice using molecular marker-assisted selection.

Agaricus blazei Murill (ABM) also known as Brazilian mushroom, originated in Brazil and Peru, is a kind of edible and medicinal fungus. It is neutral in nature and sweet in taste, distributed in heart, lung, liver and kidney channels. It has high nutritional value and medical efficacy and is rich in protein, polysaccharides, vitamins, minerals and unsaturated fatty acids. Its pharmacological ingredients mainly include nucleic acid, exogenous lectin, sterols, fatty acids and polysaccharides. It also has the effect of anti-cancer, anti-tumor, immune regulation, anti-inflammatory activity, protection of liver and kidney, treatment of diabetes, antioxidant activity, anti-fatigue effect, prevention and treatment of leukemia, asthma, and radiation injury. It can strengthen health and promote hematopoiesis. With the improvement of people’s living standards and the advancement of science and technology, many diseases already have better treatments and the average life expectancy is greatly extended. With the development of food industry, medicine and other related industries, some foods with unique medicinal effects and values such as glossy ganoderma, cordyceps sinensis and Agaricus blazei Murill have been re-recognized and used. The use of these pharmacological foods for disease prevention and treatment has become a trend. For example, the nutrients of relevant foods are extracted to make health-care products and patients are given corresponding solution to assist their treatments. Some progress has been made in realizing the pharmacological effects and dietary applications of Agaricus blazei Murill. This paper will analyze and summarize the results of current researches of pharmacological effect and application in dietotherapy of Agaricus blazei Murill, which is helpful to improve people’s understanding of its medicinal effects and to provide rich experience in dietary therapy and experimental basis. With the further research on the pharmacological effects of Agaricus blazei Murill, it will be more widely used in food therapy.

The study was carried out to characterize Ethiopian smallholder farmers’ practice and indigenous knowledge on bull station service and adoption of different mating systems. A total of 62 respondents were selected from the smallholder dairy farming communities representing three districts in the Oromia region. A multistage sampling procedure was used to select sampling areas and respondents. A standardized questionnaire was used to collect qualitative and quantitative data using person to person interview. Data were analyzed using descriptive statistical procedures of SPSS. According to the findings of the study, Ethiopian smallholder dairy farmers are using both bull service and artificial insemination for dairy cattle breeding. The bulls used for mating are either those bulls kept for communal use at bull stations or bulls belong to individual farmers. The sources of all those bulls were research centers (15.5%), NGOs (37.5%), cooperatives (7.8%), born and grown at farmers’ own farms (10%) and other sources (29.2%). About 79% of the interviewed respondents were participants of bull station service and above 63% of them started to participate in less than a decade ago. In bull station, one bull did serve 3 to 30 females per week. In addition to the bull service, considerable numbers of farmers were using artificial insemination. The study revealed that both natural mating and artificial insemination methods are acceptable as appropriate mating systems and have their own advantage and disadvantages. So, bull service can be further adopted as an option for improving dairy production and productivity at smallholder farmers’ level particularly in areas where there is limited access for an efficient AI service. The study provided valuable information that can support the importance of using bull station services and adopt different mating systems to improve dairy cattle production and productivity in smallholder dairy farming systems in Ethiopia.

The impact of nutrient sources cannot be over emphasised in plant growth. Seedlings of Annona muricata were subjected to organic fertilizers, inorganic fertilizers and fertilizer-free treatmentsin this study. The organic fertilizers comprises of cow dung, poultry manure and water hyacinth, while NPK fertilizer was used as the inorganic manure and Top soil without fertilizer application was used as the control. The organic fertilizers were singly used and mixed at the rate of 100kg/ha and 200kg/ha while the inorganic fertilizer was used at the rate 100kg/ha and 200kg/ha. The parameters taken were plant height, stem girth, leaf area and leaf production. Results obtained showed that poultry manure produced the tallest plants (23.50cm) at 3months after transplanting though this was not significantly different from those of cow dung manure (23.00cm). NPK fertilizer produced the plant with highest leaf area (32.75cm) and stem girth (2.25cm) at 3 months after transplanting and was not significantly different from the organic fertilizers (Poultry manure 200g/ha, Cow dung 200g/ha, Water hyacinth 100g/ha and 200g/ha). NPK fertilizer also produced the highest number of leaves at the end of the three month assessment (31.00cm). All the growth parameters observed showed that the control experiment gave the least performance. The results obtained tend to suggest that the use of NPK fertilizer should be recommended for growing Annona muricata and improvement of the soil nutrients level.

The presence of sodium Lauryl sulfates (SLS) as synthetic detergents, in marine environment arises mainly from its presence in complex domestic and industrial effluents as well as its release directly from some applications (e.g. oil dispersants and pesticides). It has been reported that SLS is toxic and affects survival of aquatic animals such as fishes, microbes like yeasts and bacteria. It is also toxic to mammals like mice and humans but to a lesser extent. Many studies have shown that the direct discharge of untreated wastewater into aquatic environment containing anionic surfactants causes significant damage to the aquatic environment due to the growth of algae that consume oxygen needed for the life of aquatic organisms. These damages in the aquatic environment are caused not only by the presence of anionic surfactants (SLS), but also by their interaction with other pollutants in the aquatic environment, which increases their toxic effect on aquatic organisms. Such detergents, however, became a public nuisance because they were neither soluble nor biodegradable. The present study intended to evaluate the fate of SLS as synthetic detergents on Tripoli coastal site of Libya, and quantifying their impacts on fish (Sardine aurita) DNA mutation, compared with other species through literature review. Our results provide functional evidence for genome toxicity of fish, of the DNA mutation caused by detergents effluents. This study provides the rationale for a simple genetic test to identify the impacts of detergents on aquatic ecosystem of Libyan coastal region.

The aqueous ethanolic extracts of Vernonia amygdalina, Ocimum gratissimum, Cymbopogon citrates and Annona muricata were screened for antibacterial activities at 200mg/ml concentration. Antibacterial efficacy of extracts against the bacteria were identified by gram staining techniques, coagulase and catalase tests and the bacterial isolate was indicated by the appearance of clear zones of inhibition around the disks. The extracts of Vernonia amygdalina and Ocimum gratissimum showed inhibitory activities against the bacterial isolate with zones of inhibition of 12mm and 8.5mm respectively while extracts of C.citratus and A.muricata showed no zone of inhibition. Result of the antimicrobial activity of the extracts compared effectively with the activity of standard fumigant with extract of V. amydalina showing the highest potency which was used in preparation of a local fumigant. The minimum inhibitory concentration of V.amygdalina and O.gratissimum were 25mg/ml and 50mg/ml respectively which were not determined in C.citratus and A.muricata. In conclusion, the results of this investigation showed that the aqueous ethanolic leaf extracts of V. amygdalina and O. gratissimum can be used as an effective control measure for the elimination of citrus canker of Citrus sinensis.

Industrial and municipal wastewater typically contains metal ions. When these metal ions are more than allowed, they can be harmful to aquatic organisms and human health. Environmental pollution by heavy metals is one of the main environmental problems. In this study, microbial nano cellulose was used as adsorbents in removing copper from wastewater. The purpose of this study was to study the possibility or impossibility of removing heavy metal copper by microbial nano cellulose under various environmental conditions. Removal of copper under different conditions was investigated by microbial and dry microbial nano cellulose adsorbent, contact time of 30 and 60 min and ambient temperature and temperature of ?60?^?. For further investigation, flame atomic absorption spectroscopy and infrared spectroscopy as well as scanning electron microscopy were used to illustrate the structure of microbial nano cellulose. The results showed that microbial nano cellulose are suitable for the development of economical and efficient adsorbents to remove heavy metals from the aquatic environment. According to the results, the initial metal content in the solution was 1.83 ppm, which increased with increasing temperature and time of absorption. Comparison between microbial nano cellulose and dry microbial nano cellulose. The results showed that absorption in the microbial cellulose nanoparticles is more than dry, due to the microbial cellulose structure. Also, using infrared spectroscopy, microbial nano cellulose absorption bands alone and dried microbial nanoclayers were compared with each other in a heavy metal solution, and no new absorption bar was created. As a result, the absorption of microbial cellulose nanoparticles was better at higher temperatures and more time than the rest.

The assessment of genetic diversity among 23 sesame genotypes (Sesamum indicum L.) obtained from different locations across 10 states in Nigeria was carried out using Random Amplified Polymorphic DNA (RAPD) technique. The field trial tests were carried out on the 23 sesame accessions for two seasons to have uniform genotypes from each accessions. A standard protocol of CTAB with slight modifications was employed for DNA extracted from the harvested seeds. The extracted DNA samples were observed under UV illumination using agarose gel electrophoresis after staining with ethidium bromide. A total of 7 primers were used for PCR amplification, 5 of which have been previously used to discriminate sesame genotypes from other countries. Only 3 of the 7 primers considered produced strong amplification with the selected 23 sesame samples. A total of 47 amplified products were produced by the 3 primers among the 23 accessions all of which are 100% polymorphic. The estimates of similarity index for the 23 accessions ranged from 0.29 to 0.92. Cluster analysis revealed 2 main clusters with some of the accessions from different geographical origin cluster together in the same group which might indicate the involvement of human factor in the spread of sesame varieties in Nigeria. The relevance of RAPD to this study was evident from the high level of polymorphism reported in this study. There is therefore existence of adequate genetic diversity among the 23 Nigerian sesame accessions for sesame breeders to develop improved varieties.

Pasta are present in the basic basket of the Brazilian population and constitute one of the main substitutes for rice. The ingredient traditionally used in the preparation of these pastas is wheat flour, in which gluten is present. Gluten is proven to be related to some diseases that have been increasingly common in our population. The aim of this work was conduct an integrative review on the main wheat substitutes in the preparation of gluten-free pasta. For this, the following question arose: what are the current substitutes for wheat in the preparation of gluten-free pasta? The survey was conducted from January to June 2019, in the main electronic databases and online platforms. 84 articles were found, and 14 articles were included because they were eligible. Thirteen different raw materials were found, used in the preparation of noodles, rice being the most prevalent in substitution to wheat. The range of ingredients that can be used for wheat substitution is evident, but it is expected that further studies will be carried out in order to be able to produce pasta with characteristics closer to those offered by gluten and that are sensorial accepted by the population.

Lipids correlate with membrane characteristics and functionalities as macromolecular constituentts in all cellular processes. Numerous aspects of lipid modulation of protein activity and structure are not completely understood and, thus a holistic systematic investigation activities will be pertinent. Protein-lipid interactions are the resultant impacts of membrane proteins on lipid physical states or vice versa. Encompassing research needs to be associated with strategies to elucidate whether proteins contain binding sites which are lipid specific, and that the protein-lipid complexes are ostensibly long-lived, on the time order necessary for the turnover of a normal enzyme. Biological membranes have since been determined as essential ingredients in an expansive array of cellular processes, such as photosynthesis, cell defence, signaling transduction, communication and motility. Therefore, they constitute multiple targets in both basic and applied research. Protein-lipid interactions are becoming increasingly relevant to the morphological characterization of membrane proteins as related to their functionalities. Excepting for simplified models, certain protein-lipid interactions specifically constitute remarkable challenges which require optimum experimental paradigm and design.

This paper attempts to review available pertinent scientific and medical literature that the current global epidemic of obesity and diabetes has increased simultaneously or contemporaneously with adverse metabolic events. There is widespread established evidence that the type of carbohydrate consumed is important in the development or prevention of insulin resistance, obesity, and the metabolic syndrome. There is scarcity of published data on the combined effects of interactions between micronutrients, carbohydrates and lipids. However, there are extensive data supporting the benefits of the effects of both carbohydrates and lipids on various mechanisms of energy balance and diseases. Adequate measure is necessary to control carbohydrate-lipid interactions as they impact on obesity and diabetes.

This work investigates the growth and physiological responses of locally grown tomato cultivars with a known hybrid (Roma-VF cultivars). Seeds of two tomato cultivars: Roma VF (Lycopersicum esculentum Mill.Cv) was collected from National Horticulture Research Institute (NIHORT), Ibadan while the Local tomato: Ife-1 (Lycopersicum esculentum Mill. Cv) obtained from the market woman in the central market, Obafemi Awolowo University, Ile- Ife. The seed of the plant were planted in perforated plastic containers containing soil collected at the base of hill 1(latitude 7’3’9.40 and longitude 4’3’24.52) in Obafemi Awolowo University Ile-Ife, Osun State. The containers placed where they can have access to direct sunlight. The growth parameters of the plants were determined which include the leaf length, width and the shoot size was observed. The weight and growth rate of the shoot were determined. The fruits obtained from all the cultivar were exposed to proximate analysis. It was observed that the shoot of the two cultivars increased in the course of the experiment but at the end the shoot of Roma () was longer than that of local cultivar. More number of leaves was present in Roma compare to local one. Roma has higher moisture content (5.9% M.C)) than the local cultivar (4.7% M.C). Average number of fruits in Roma was (15) which is higher than that of local one (10). From all the parameters observed in this work it was observed that Roma cultivar which is a hybrid do far better in all indication than the local tomato cultivar. This may be as a result of Roma hybrid characteristics such as resistances to disease and unfavorable environmental factors. Equally the improved nutritional value of the Roma tomato and lot of genetically modified features are key to this cultivar (Roma) advancement.

In an experiment to determine the effect of feeding graded levels of Ipomoea asarifolia Leaf Meal (IALM) on the growth, heamatology and serum chemistry of rabbits in the tropics, twenty-four (24) weaned male rabbits, 6-8 weeks old, were randomly allotted to four (4) dietary treatments in which IALM was used to replaced soybean meal (SBM) at 0% (control), 10%, 20%, and 30% with six (6) rabbits per treatment in a completely randomized design experiment. Each rabbit constituted a replicate. Feed and water were offer ad libitum while other standard management practices were meticulously observed. The Proximate analysis of the diets and leaf was determined according to the AOAC [1] for crude protein, crude fibre, ether extract (fat) and ash while haematological analysis was done using Sysmex KX-21N automated analyzer and while serum parameters were analyzed following standard procedure. The data obtained were subjected to one-way analysis of variance in a completely randomized design experiment. The IALM had average crude fibre percentage of 7.15%, while crude protein, ash, ether extract and metabolisable energy were 28.40%, 11.00%, 7.10%, 3236.15 KcalKg) respectively. All growth parameters were affected by IALM except weight gained and FCR. Haematological and serological parameters measured at the end of 8 weeks of feeding indicated significant (P<0.05) variations in, mean cell haemoglobin (MCH) and alkaline phosphatase, cholesterol, globulin, platelet, white blood cell count (WBC) and total serum proteins with variations in the level of dietary IALM. The aspartate amino acid, alanine amino acid, red blood cell count, mean cell volume (MCV), and intermediate cell count were, however, unaffected (P>0.05) by variations in the level of dietary IALM.

Physalis pubescens L. has exotic fruit, native to the Americas, with potential to be widely used for consumption. This plant has yellow fruits accompanied by chalices. The aerial parts are used in traditional medicine due to its preventive and curative effect. Its fruits are aromatic and mildly acidic, besides containing compounds that contribute to the functional capacity of the fruit. Due to the health benefits, the use of this plant has generated a great commercial interest. Purpose of this review was to compile information on the functional properties of Physalis pubescens, as well as to explore information on some of the major species of Physalis.

This study is aimed at evaluating the food vendor’s practices, proximate analysis of locally prepared vended dishes in Owerri Municipality of Imo state. Five zones (A, B, C.D and E) were considered and the food assessed were cooked spaghetti, beans, rice and stew. The proximate analysis of the food samples revealed that the mean moisture content ranged from 59.91% in cooked spaghetti to 89.0% in stew. Protein content varied from 2.23% in stew to 13.45% in cooked beans, while Fat varied from 0.13% in cooked rice to 8.02% in stew; Ash ranged from 0.22% in cooked beans to 0.81% in stew; Carbohydrate ranged from 17.73% in cooked beans to 36.33% in cooked spaghetti; the Nitrogen Free Extract (NFE) present only in stew ranged from 0.43% in Zone E to 3.28% in Zone A. It was concluded that street foods provide a source of affordable nutrients to the majority of the people especially the low-income group in the developing countries, however, all the proximate components were within the normal range in the zones.